Apoe-KO(2)/hANGPTL3(4)

Fig1. The body weight of male WT mice and Apoe-KO(2)/hANGPTL3(4) mice under chow diet conditions (n=3 for each group). There were no significant differences in body weight or body weight gain between Apoe-KO(2)/hANGPTL3(4) mice and WT mice.

Abbr. WT, wild-type; HO, homozygous.

Fig2. Blood biochemical test of hepatic function in male WT mice and Apoe-KO(2)/hANGPTL3(4) mice under chow diet conditions. There were no significant differences in ALT or AST levels between Apoe-KO(2)/hANGPTL3(4) mice and WT mice, with both markers varying within a similar range between the two groups. Serum samples were collected following a 6-hour fast to measure levels of (A) aspartate aminotransferase (AST), (B) alanine aminotransferase (ALT) (n=3 for in each group, Mean ± SEM, T-test).

Abbr. WT, wild-type; HO, homozygous.

Fig3. Blood lipid profile of male WT mice and Apoe-KO(2)/hANGPTL3(4) mice under chow diet conditions. Apoe-KO(2)/hANGPTL3(4) mice exhibited significantly elevated serum TC and LDL-C levels compared to WT controls, and showed trends toward to higher TG levels and lower HDL-C levels, indicating lipid dysmetabolism in Apoe-KO(2)/hANGPTL3(4) mice. Serum samples were collected following a 6-hour fast to measure levels of (A) triglycerides (TG), (B) total cholesterol (TC), (C) low-density lipoprotein cholesterol (LDL-C), and (D) high-density lipoprotein cholesterol (HDL-C). (n=3 for in each group, Mean ± SEM, T-test, *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001).

Abbr. WT, wild-type; HO, homozygous.

Fig4. Detection of serum hANGPTL3 protein levels in Apoe-KO(2)/hANGPTL(4) mice under chow diet conditions. hANGPTL3 was detectable exclusively in homozygous Apoe-KO(2)/hANGPTL3(4) mice but not in WT mice. Serum hANGPTL3 levels in Apoe-KO(2)/hANGPTL3(4) mice were comparable across different ages. Serum samples were collected following a 6-hour fast to measure hANGPTL3 levels using an ELISA kit (n=3 in each group, Mean ± SEM). 

Abbr. WT, wild-type; HO, homozygous.

Fig5. Oil Red O staining of aortic valves in male WT mice and Apoe-KO(2)/hANGPTL3(4) mice under chow diet conditions (n=3 in each group, 20 weeks old). No lipid accumulation was observed in the aortic valves of WT mice at 5 months of age. In contrast, all Apoe-KO(2)/hANGPTL3(4) mice exhibited significant lipid accumulation in aortic valves at 5 months of age.

Abbr. WT, wild-type; HO, homozygous.

Fig6. Oil Red O staining of aortas in male WT mice and Apoe-KO(2)/hANGPTL3(4) mice under chow diet conditions (n=3 in each group, 20 weeks old). No lipid accumulation was observed in aortas of WT mice at 5 months of age. In contrast, Apoe-KO(2)/hANGPTL3(4) mice exhibited significant lipid accumulation near the aortic arch at 5 months of age.

Abbr. WT, wild-type; HO, homozygous.