Lep-KO

品系全名

B6;129S-Leptm1Smoc

目录号

NM-KO-00034

品系状态

活体

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基因信息

基因名
Lep

基因曾用名

ob; obese

NCBI ID

16846

MGI ID

104663

Ensembl ID

ENSMUSG00000059201

Pubmed

Lep

人类同源基因

LEP

品系描述

瘦素基因缺失小鼠是研究胰岛素抵抗、肥胖、II型糖尿病等疾病的重要工具。瘦素基因的突变或缺失会导致严重的肥胖、肥胖引起的性腺功能不足，并与II型糖尿病的发展密切相关。研究还发现瘦素与心血管功能相关。该小鼠通常也称为ob/ob小鼠
应用领域：胰岛素抵抗、肥胖、II型糖尿病等

疾病预测

2型糖尿病
Type 2 Diabetes Mellitus

近似模型的表型

MGI:5428893

参考文献

Clee SM, Nadler ST, Attie AD, Genetic and genomic studies of the BTBR ob/ob mouse model of type 2 diabetes. Am J Ther. 2005 Nov-Dec;12(6):491-8

非酒精性脂肪肝
Non-Alcoholic Fatty Liver Disease

近似模型的表型

MGI:5807153

参考文献

Trak-Smayra V, Paradis V, Massart J, Nasser S, Jebara V, Fromenty B, Pathology of the liver in obese and diabetic ob/ob and db/db mice fed a standard or high-calorie diet. Int J Exp Pathol. 2011 Dec;92(6):413-21

肥胖
Obesity

近似模型的表型

MGI:3623749

参考文献

Barouch LA, Berkowitz DE, Harrison RW, O'Donnell CP, Hare JM, Disruption of leptin signaling contributes to cardiac hypertrophy independently of body weight in mice. Circulation. 2003 Aug 12;108(6):754-9

腹部肥胖代谢综合征 1
Abdominal Obesity-Metabolic Syndrome 1

近似模型的表型

MGI:2654709

参考文献

Ikels K, Kuschel S, Fischer J, Kaisers W, Eberhard D, Ruther U, FTO is a relevant factor for the development of the metabolic syndrome in mice. PLoS One. 2014;9(8):e105349

腹部肥胖代谢综合症
Abdominal Obesity-Metabolic Syndrome

近似模型的表型

MGI:4429407

参考文献

Xu A, Liu J, Liu P, Jia M, Wang H, Tao L, Mitochondrial translocation of Nur77 induced by ROS contributed to cardiomyocyte apoptosis in metabolic syndrome. Biochem Biophys Res Commun. 2014 Apr 18;446(4):1184-9

验证数据

image.png

Fig1. Body weight and Blood glucose monitoring of ob/ob mice.

image.png

Fig2. Evaluation of Oral glucose tolerance test (OGTT) of female ob/ob mice. 

Abbr. HO, homozygous; WT, wild type. **, P ≤ 0.01.

Note. The tested ob/ob and C57BL/6 mice were 6 weeks old.

image.png


Fig3. Detection of insulin levels in serum by ELISA. 

Abbr. Hom, homozygous; HE, heterozygous; WT, wild type.

Note. The tested ob/ob and C57BL/6 mice were 20 weeks old.

image.png

Fig4. H&E staining of the subcutaneous adipose tissue (SAT) in ob/ob mice. 

Abbr. HO, homozygous; WT, wild type; WAT, white adipose tissue; BAT, brown adipose tissue. 

Note. The tested ob/ob and C57BL/6 mice were 20 weeks old. Scale bar, 200 μm; magnification, × 20.

image.png

Fig5. Representative pictures of pancreas in ob/ob mice. 

These results suggested the inflammatory cell infiltration of the pancreas in ob/ob mice. 

Abbr. HO, homozygous; WT, wild type. 

Note. The tested ob/ob and C57BL/6 mice were 20 weeks old. Scale bar, 200 μm; magnification, × 10.

发表文献 5篇

1. DYRK1B phosphorylates FOXO1 to promote hepatic gluconeogenesis
发表年份：2025 来源杂志：NUCLEIC ACIDS RESEARCH

2. Identification of CDKN1A as a potential key risk factor in MASLD progression
发表年份：2025 来源杂志：FASEB JOURNAL

3. Deficiency of hepatokine orosomucoid1 aggravates NAFLD progression in mice
发表年份：2025 来源杂志：BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE

4. The deubiquitinating enzyme 13 retards non-alcoholic steatohepatitis via blocking inactive rhomboid protein 2-dependent pathway
发表年份：2022 来源杂志：Acta Pharmaceutica Sinica B

5. Pancreatic β cell microRNA-26a alleviates type 2 diabetes by improving peripheral insulin sensitivity and preserving β cell function
发表年份：2020 来源杂志：PLOS BIOLOGY

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